Secretion is a principle function of a cell. Neurotransmitter and hormone secretion is triggered by increase in intracellular Ca concentration. We are interested in mechanisms of how intracellular Ca is regulated in single cell level by advanced methods including biophysics (patch-clamp, CFE amperometry, confocal, multi-photon, TIRFM, optogenetics, EM), biochemistry and molecular biology. We investigate mechanisms of neurotransmitter, in particular catecholamines (dopamine, noradrenalin, serotonin), release from soma (or synapse) of single cells (DRG neuron, astrocyte, chromaffin cell), brain slices (dopamine neurons, locus coeruleus, Me5) or living rat/mouse brains (striatum, SNc/VTA, hippocampus) using animal disease models (PD, HD, schizophrenia, cocaine/ nicotine-addiction). We also study mechanisms of endocytosis. We develop /modifying biophysical technologies (probes, preparations, assay methods) for secretion, ion channels, Ca homeostasis and imaging. Our goal is to discover novel mechanisms of cell secretion in healthy and diseases conditions. For example, we discovered Ca independent but voltage dependent secretion (CIVDS) in DRG neurons (2002-); kiss-and-run secretion in chromaffin cells (1996, 2005); kiss-and-run exocytosis in astrocytes (2005, 2011).
 
Lab Members:
E-mail: zzhou@ pku.edu.cn
Lab manager and PI assistant: Lianghong Zheng
E-mail: lhzheng@ pku.edu.cn 
Secretary: Han Ge
E-mail: hange@pku.edu.cn
Postdoctor: Changhe Wang
Technicans: Yangjuan Wang, Hezheng Xu
Tel: 86-10-6276-7850, 
  
Graduate Students: Sasa Teng,Meiqing Hu, Xinjiang Kang, Bin Liu, Qihui Wu, Huadong Xu, Xiaoyu Zhang, Feipeng Zhu, Zuying Chai, Mingli Li, Li Zhou, WeiLiu, Bin Liu, Quangfeng Zhang, Ruiying Jiao, Xiaoxuan Sun
Visiting scientists/students:Yaoxu Guo, Rong Huang 
Guest investigators: Dr. Luyang Wang(University of Toronto), David Z. Xu (Karolinska Institute), Dr. Weiping Han (Singapore Bioimaging Consortium, Agency for Science, Technology, and Research), Dr. Hongbo Jia (Technische Universitaet Muenchen)


Lab Address:Room 336, Ying-Jie Conference Center, Peking University 
Tel:86-10-62767850 
Post Address:Room 336, Ying-Jie Conference Center, Peking University, Beijing, China
Postal Code: 100871