Zhuan Zhou, Ph.D.

Professor, Senior Principal Investigator,

Laboratory of Cellular Biophysics and Neurodegeneration

Room 324, Ying-Jie Conference Center, Peking University

Beijing, China 100871

E-mail: zzhou@ pku.edu.cn

Phone: 86-10-6276-7850
 

Zhuan Zhou, Ph.D., Principle Investigator of Laboratory of Cellular Biophysics. 1984, B.S. Electronic instrumentation, Tongji University, Shanghai. 1990, Ph.D. Biomedical Engineering, Huazhong University of Science and Technology (HUST), Wuhan. 11/ 1990-2/1993, postdoctoral fellow in Erwin Neher's lab, Max-Planck-Institute for Biophysical Chemistry, Goettingen, Germany. 1993-95, Research Instructor, Departments of Physiology, Washington University. St. Louis, USA. Nov. 1995-97, Researcher Assistant Professor, Department of Physiology, Loyola University, Chicago, USA. 1997.9-1999.9, professor and head, Department of Neuroscience and Biophysics, University of Science and Technology of China, Hefei. 4/1993-5/2000, professor and director, 1999.10-2004.12, Principle Investigator, Institute of Neuroscience, Chinese Academy of Sciences; 2005-, Peking University. Vice president of Biophysical Society of China and chair of the Neurobiophysics and Neuroinformation Committee; Board member of International Symposium of Chromaffin Cells Biology. Editorial board member of journals: Journal of Physiology-London, NeuroMolecular Medicine, Cell Calcium.

Research Interests:

Secretion is a principle function of a cell. Neurotransmitter and hormone secretion is triggered by increase in intracellular Ca concentration. We are interested in mechanisms of how intracellular Ca is regulated in single cell level by advanced methods including biophysics (patch-clamp, CFE amperometry, confocal, multi-photon, TIRFM, optogentics, EM), biochemistry and molecular biology. We investigate mechanisms of neurotransmitter, in particular catecholamines (adrenalin, noradrenalin, dopamine, serotonin), release from soma (or synapse) of single cells (DRG neuron, glial, chromaffin cell, pancreatic B cell), brain slices (dopamine neurons, locus coeruleus, Me5) or living rat/mouse brains (striatum, nuclei accubens, hippocampus) using animal disease models (PD, HD, schizophrenia, cocaine/ nicotine-addiction). We also study mechanisms of endocytosis. We create/modify biophysical technologies and micro probes for Ca homeostasis and secretion. Our goal is to discover mechanisms of cell secretion in healthy and diseases conditions.

 

Selected publications:

英文论文：

109. Huang R, Wang Y, Li J, Jiang X, Li YL, Liu B, Wu X, Du X, Hang Y, Jin M, Zhu F, Wang C, Chai Z and Zhou Z (2019) Ca2+-Independent but Voltage-Dependent Quantal Catecholamine Secretion (CiVDS) in the Mammalian Sympathetic Nervous System. PNAS, pii: 201902444. doi:10.1073/pnas.1902444116. [Epub ahead of print].

108. Li M, Xu H, Chen G, Sun S, Wang Q, Liu B, Wu X, Zhou L, Chai Z, Sun X, Lu Y, Younus M, Zheng L, Zhu F, Jia H, Chen X, Wang C, Zhou Z. (2019) Impaired D2 receptor-dependent dopaminergic transmission in prefrontal cortex of awake mouse model of Parkinson's disease. Brain,142:3099-3115. Preprint doi:10.1093/brain/awz243 BRAIN.

107. Zhang QF, Liu Bin, Wu QH, Liu Bing, Li YL,  Sun SH, Wang Y, Wu X, Chai ZY, Jiang XH, Liu XY, Hu MQ, Wang YS, Yang YT, Wang L, Kang XJ, Xiong YF, Zhou Y, Chen XK, Zheng LH, Zhang B, Wang CH, Zhu FP and Zhou Z (2019) Differential controls of two native transmitters from a vesicle fusion pore in adrenal chromaffin cells. Neuron，102：173-183

106. Wu HK, Zhang Y, Cao CM, Hu X, Fang M, Yao Y, Jin L, Chen G, Jiang P, Zhang S, Song R, Peng W, Liu F, Guo J, Tang L, He Y, Shan D, Huang J, Zhou Z, Wang D, Lv F, Xiao RP. (2019) Glucose-sensitive Myokine/Cardiokine MG53 Regulates Systemic Insulin Response and Metabolic Homeostasis. Circulation, 139(7):901-914. doi:10.1161/CIRCULATIONAHA.118.037216.

105. Wu QH, Zhang QF, Liu B, Li YL, Wu X, Kuo ST, Zheng LH, Wang CH, Zhu FP and Zhou Z* (2018) Dynamin-1 restrains vesicular release to a sub-quantal mode in mammalian adrenal chromaffin cells. Journal of Neuroscience, 39(2):199 -211.

104. Zhuan Zhou*, Zuying Chai, Changhe Wang (2018) From structure to function: unveiling the structure of Nav channel-toxin complex. National Science Review, nwy090, https://doi.org/10.1093/nsr/nwy090, Published: 14 September 2018

103. Xiong Y, Sun S, Teng S, Jin M* and Zhou Z* (2018) Ca2+-Dependent and Ca2+-Independent ATP Release in Astrocytes. Front. Mol. Neurosci. 11:224 (pp1-5). doi: 10.3389/fnmol.2018.00224

102. Dou HQ* and Zhou Z* (2018) Action potential-induced Ca2+ influx for both acute and sustained insulin secretion in pancreatic beta cells. In: Lemos J and Dayanithi G (Eds) International Neuroendocrine Federation (INF) Master class in Neuroendocrinology Series Neurosecretion: Secretory Mechanisms. Chapter 8. Springer, in press.

101. Bingying Chen, Xiaoshuai Huang, Dongzhou Gou, Jianzhi Zeng, Guoqing Chen, Meijun Pang, Yanhui Hu, Zhe Zhao, Yunfeng Zhang, Zhuan Zhou, Haitao Wu, Heping Cheng, Zhigang Zhang, Chris Xu, Yulong Li, Liangyi Chen, and Aimin Wang (2018) Rapid volumetric imaging with Bessel-Beam three-photon microscopy. Biomedical Optics Express, 9: 1992-2000

100. Xiong Y., Teng S., Zheng L., Sun S., Li J., Guo N, Li ML, Wang L, Zhu F, Wang C, Rao Z., and Zhou Z*. (2018). Stretch-induced Ca2+-independent ATP release in hippocampal astrocytes. Journal of Physiology, 596: 1931–1947. PMID: 29488635 DOI: 10.1113/JP275805

99. Liu B, Wu QH, Wang YS, Hu MQ, Zhu FP, Zheng LH, Wang CH and Zhou Z (2017) Differential controls of two native transmitters from a vesiclefusion pore in adrenal chromaffin cells. Neuron, in revision.

98. Yingfei Xiong, Sasa Teng, suhua Sun, Huadong Xu, Ning Guo, Yang Zhou, Tao Liu, Li Wang, Xiaoke Chen, Bin Liu, Changhe Wang, Lianghong Zheng, Jin Lu, Claire Xi Zhang, Zhiren Rao and Zhuan Zhou (2017) Direct recording of non-vesicular ATP release from a hippocampal astrocyte. J Physiol, 2^nd in revision.

97. Wang CH, Kang XJ, Chai ZY, Wu QH1, Huang R, Hu MQ, Liu B1, Zhu FP, Zhang QF, Zhou L, Jiao RY, Xu HD, Shang SJ, Zuo PL, Wang L, Zheng LH, Yue ZY, and Zhou Z (2017) Synaptotagmin-11 mediates parkin-linked pathogenesis in Parkinson’s disease. Nature Communications, in press.

96. Chai ZY, Zhang XY, Huang R, Wang Y, Zhou R, Wu QH, Hu MQ, Wu X, Liu HW, Liu T, Wang YS, Guo W, Zheng H, Xiong W, Zhang C, Zheng LH, Zhang CX, Ding JP, Wang CH, Zhou Z (2017) CaV2.2 Gates Calcium-Independent but Voltage-Dependent Secretion in Mammalian Sensory Neurons, Neuron, 96：1-10. https://doi.org/10.1016/j.neuron.2017.10.028; Highlighted by 4 Commentaries: (1) Natasha B (2017) Nature Review Neuroscience, doi:10.1038/nrn.2017.162; (2) Yang Y (2017) F1000Prime DOI: 10.3410/f.732224032.793540321; (3) Holz R (2018) F1000Prime DOI: 10.3410/f.732224032.793541931; (4) Colmers W (2018) F1000Prime DOI: 10.3410/f.732224032.793542130.

95. Feipeng Zhu, Qihui Wu, Jie Li, Katarzyna Grycel, Bing Liu, Xiaoxuan Sun, Li Zhou, Ruiyin Jiao, Rui Song, Younus M. Khan, Qinglong Wang, Lun Wang, Yongxin Xu, Jin Li, Bo Zhang, Zhuan Zhou (2017) A single dose of cocaine potentiates glutamatergic synaptic transmission onto locus coeruleus neurons. Cell Calcium (IF 4), 67 : 11-20.

94. Chae-Seok Lim, Cheng Wen, Yanghui Sheng, Guangfu Wang, Zhuan Zhou, Shiqiang Wang, Huaye Zhang, Anpei Ye and J. Julius Zhu (2017) Piconewton-scale analysis of Ras-BRaf signal transduction with single-molecule force spectroscopy. Small (IF 8.3), in press

93. Wang YS, Wu QH, Hu MQ, Liu B, Chai ZY, Xu HD, Zhou L, Zheng LH, Wang CH, Zhou Z (2017) Ligand-gated and voltage-gated calcium channels differentially regulate “kiss-and-run” vesicular neuropeptide release in mammalian sensory neurons. Science Signaling, 10(484) pii: eaal1683. doi: 10.1126/scisignal.aal1683. Highlighted by Ferrarelli LK. (2017) Kiss-and-run or a full commitment?, Science , 356(6344):1243-1245. doi: 10.1126/science.356.6344.1243-p.

92. Zong W#, Wu R#, Li M#, Wu H, Jia H, Fan M, Zhou Z, Zhang Y, Wang A, Chen L, Cheng H (2017) Fast High-resolution Miniature Two-photon Microscopy for Brain Imaging in Freely-behaving Mice. Nature Methods, 14(7):713-719. doi: 10.1038/nmeth.4305. (#equal contributors, this “Engineering + Biology” work, we did biology)

91. Yu-Zhen Chen*, Xiao-Cun Li, Zhenquan Guo, Li Zhou, Zhuan Zhou*, Song-Ping Liang, Cai-Hong Wu (2017) Porcine diazepam-binding inhibitor and bovine diazepam-binding inhibitor affect morphine antinociception via different receptors. Protein & Cell, 8(2):140-143. doi: 10.1007/s13238-016-0355-5 (* co-corresponding authors)

90. Chunhua Liu, Zheng Gong, Zong-Lai Liang, Zhi-Xin Liu, Fan Yang, Yu-Jing Sun, Ming-Liang Ma, Yi-jing Wang, Chao-Ran Ji, Yu-Hong Wang, Mei Jie Wang, Fu-Ai Cui, Amy Lin, Wen-Shuai Zheng, Dong-fang He, Chang-xiu Qu, Peng Xiao, Chuanyong Liu, Alex Thomsen, Thomas Cahill III, Alem Kahsai, Fan Yi, Kunhong Xiao, Tian Xue, Zhuan Zhou, Xiao Yu, and Jinpeng Sun (2017) Arrestin biased AT1R agonism induces acute catecholamine secretion through TRPC3 coupling. Nature Communications, 8:14335

89. Shang SJ, Zhu FP, Liu B, Chai ZY, Wu QH, Hu MQ, Wang Y, Huang R, Zhang XY, Wu X, Sun L, Wang Y, Wang L, Xu HD, Teng SS, Liu B, Zheng LH, Zhang C, Zhang F, Feng X, Zhu DS, Wang CH, Liu T, Zhu MX, Zhou Z(2016) Intracellular TRPA1 mediates Ca2+ release from lysosomes in dorsal root ganglion neurons. J Cell Biol , 215(3):369-381. DOI:10.1083/jcb.201603081. Highlighted by Gu M and Xu HX (2016) A painful TR(i)P to lysosomes, J Cell Biol , 215(3):309-312 DOI: 10.1083/jcb.201610067

88. Zhang K, Chen C, Yang Z, He W, Liao X, Ma Q, Deng P, Lu J, Li J, Wang M, Li M, Zheng L, Zhou Z, Sun W, Wang L, Jia H, Yu Z, Zhou Z, Chen X (2016) Sensory Response of Transplanted Astrocytes in Adult Mammalian Cortex In Vivo. Cerebral Cortex, 26:3690-704

87. Wang CH, Wang YS, Hu MQ, Chai ZY, Wu QH, Huang R, Han WP, Zhang CX and Zhou Z (2016) Synaptotagmin 11 inhibits clathrin-mediated and bulk endocytosis. EMBO Reports, 17(1):47-63. DOI: 10.15252/embr.201540689

86. Shang CF, Li XQ, Yin C, Liu B, Wang YF, Zhou Z*, Du JL* （2015）Amperometric Monitoring of Sensory-Evoked Dopamine Release in Awake Larval Zebrafish. J Neurosci. 35(46):15291-4. doi: 10.1523/JNEUROSCI.3050-15.2015

85. He LL, Wang LC, Dai JX, and Zheng LH, Zhou Z (2015) Muscarine inhibition of Nicotinic Transmission in Superior Cervical Ganglion Neurons. Philosophical Transactions B, 370(1672). pii: 20140188. doi: 10.1098/rstb.2014.0188.

84. Xu H, Zuo P, Wang S, Zhou L, Sun X, Hu M, Liu B, Wu Q, Dou H, Liu B, Zhu F, Teng S, Zhang X, Wang L, Li Q, Jin M, Kang X, Xiong W, Wang C, Zhou Z (2015) Striatal dopamine release in a schizophrenia mouse model measured by electrochemical amperometry in vivo. Analyst, 140:3840 - 3845.

83. Hao ZH, Wei LS, Feng YQ, Chen XW, Du W, Ma J, Zhou Z, Chen LY, Li W (2015) Impaired maturation of large dense core vesicles in muted-deficient adrenal chromaffin cells. Journal of Cell Science, 128:1365–1374.

82. Dou HQ, Wang CH, Wu X, Yao LJ, Zhang XY, Teng SS, Xu HD, Liu B, Wu QH, Zhang QF, Hu MQ, Wang YS, Wang L, Wu Y, Shang SJ, Kang XJ, Zheng LH, Zhang J, Raoux M, Lang J, Li Q, Su J, Yu X, Chen LY and Zhou Z (2014) Calcium influx activates adenylyl cyclase 8 for sustained insulin secretion in rat pancreatic beta cells. Diabetologia,58:324–333

81. Kang XJ, Wang CH, Xu HD, Shang SJ, Zhang XY, Zuo PL, Zhou L, Deng ZJ, Liu B, Liu B, Wu QH, Wang L, Hu MQ, Dou HQ, Liu W, Zhu FP, Li Q, Guo S, Gu JL, Lei Q, Lü J, Jin M, Jiang W, Wang SR, Liu K, Li WL, Zhang K, Zhou Z (2014) Rescuing dopamine release and parkinsonian behavior by neural stem cells. PNAS, Epub ahead www.pnas.org/cgi/doi/10.1073/pnas.1408484111

80. Zong WJ, Zhao J, Chen XY, Lin Y, Ren HX, Zhang YF, Fan M, Zhou Z, Cheng HP, Sun YJ and Chen LY (2014) Large-field high-resolution two-photon digital scanned light-sheet microscopy. Cell Research, Epub ahead, http://www.nature.com/cr/journal/vaop/ncurrent/full/cr2014124a.html

79. Li YT, Zhang SH, Wang L, Xiao RR, Liu W, Zhang XW, Zhou Z, Amatore C, Huang WH (2014) Nanoelectrode for Amperometric Monitoring of Individual Vesicular Exocytosis Inside Single Synapses. Angew. Chem. Int. Ed., Epub ahead, DOI: 10.1002/anie.201404744

78. Yang YM, Wang W, Fedchyshyn MJ, Zhou Z, Ding JP, Wang LY (2014) Enhancing the fidelity of neurotransmission by activity-dependent facilitation of presynaptic K+ currents. Nature Communications, doi:10.1038/ncomms5564

77. Liu T, Li HY, Gounko N, Zhou Z, Xu AM, Hong WJ, and Han WP (2014) Detection of insulin granule exocytosis by an electrophysiology method with high temporal resolution reveals enlarged insulin granule pool in BIG3 knockout mice. Am J Physiol-Endo M, 307(7):E611-8

76. Shang SJ, Wang CH, Liu B, Wu QH, Zhang QF, Liu W, Zheng LH, Xu HD, Kang XJ, Zhang XY, Wang YS, Zheng H, Wang SR, Xiong W, Liu T, Zhou Z (2014) Extracellular Ca2+ per se inhibits quantal size of catecholamine release in adrenal slice chromaffin cells. Cell Calcium, 56:202–207.

75. Wang L, Zhang XY, Xu HD, Zhou L, Jiao RY, Liu W, Zhu FP, Kang XJ, Liu B, Teng SS, Wu QH, Li ML, Dou HQ, Wang CH, Zuo PL, Wang SR and Zhou Z (2014) Temporal components of synapse-synapse transmission in dorsal striatum brain slices of mice. Journal of Physiology, 592:3559–3576.

74. Wang L, Shang SJ, Kang XJ , Teng SS, Zhu FP, Liu B, Wu QH, Li ML, Liu W, Xu HD, Zhou L, Jiao RY, Dou HQ, Zuo PL, Zhang XY, Zheng LH, Wang SR, Wang CH and Zhou Z (2014) A mechanism of modulating dopamine release in the striatum by smoking-level nicotine. Nature Communications, DOI: 10.1038/ncomms4925.

73. Kuo ST, Wu QH, Liu B, Xie ZL, Wu X, Shang SJ, Zhang XY, Kang XJ, Liu LN, Zhu FP, Wang YS, Hu MQ, Xu HD, Zhou L, Liu B, Chai ZY, Zhang QF, Liu W, Teng SS, Wang CH, Guo N, Dou HQ, Zuo PL1, Zheng LH, Zhang CX1, Zhu DS, Wang L, Wang SR, and Zhou Z. (2014). Importing, caring, breeding, genotyping, and phenotyping a genetic mouse in a Chinese University. J Mol Neurosci, 53(3):487-92.

72. Liu LN, Zhu FP, Song MY, Zhang B, Kuo ST, Liu W, Li ML, Zhou L, Jiao RY, Liu B, Zhang XY, Wang CH, Wang SR and Zhou Z. (2014). Cocaine modulation of ion channels and action potentials in locus coeruleus brain slices. J Mol Neurosci, 53(3):345-51

71. Zhang XY, Wen JG, Yang W, Wang C, Gao L, Zheng LH, Wang T, Ran KK, Li YL, Li, Ming Xu XY, Luo JY, Feng SL, Ma XX, Ma HY, Chai ZY, Zhou Z, Yao J, Zhang X, Liu JY (2013) Gain-of-function mutations in SCN11A cause familial episodic pain. American Journal of Human Genetics, 93(5):957-66

70. Zhang H, Shang W, Zhang X, Gu J, Wang X, Zheng M, Wang Y, Zhou Z, Cao JM, Ji G, Zhang R, Cheng H. (2013) β-Adrenergic-stimulated l-type channel Ca(2+) entry mediates hypoxic Ca(2+) overload in intact heart. Journal of Molecular and Cellular Cardiology. 65: 51–58

69. Liang-Hong Zheng, Shujiang Zhang, Changhe Wang, Meiqin Hu, Yeshi Wang, Xiaoyu Zhang, Zuyin Chai, Xi Wu, Hui Zheng, Chen Zhang, Lie-Cheng Wang, Wei Xiong, and Zhuan Zhou. (2013) Real Time Endocytosis Imaging as Rapid Assay of Ligand-GPCR Binding in Single Cells. Am J Physiol, 305: C751–C760

68. Zuo PL, Yao W, Sun L, Kuo ST, Li Q, Wang SR, Dou HQ, Xu HD, Zhang CX, Kang XJ, Zhou Z* and Zhang B*. (2013) Impulse-dependent extracellular resting dopamine concentration in rat striatum in vivo, Neurochemistry International, 62:50-7.

67. Loh YP, Zhou Z, Kao LS. (2012) Proceedings of the 16th international symposium on chromaffin cell biology. J Mol Neurosci. 48(2):313-314.

66. Carbone E, Guérineau NC, Loh YP and Zhou Z. (2012) Commentary: Ion Channels, Fusion Pores and Exocytosis. J Mol Neurosci. 48(2):313-314.

65. Jing Lü, Xue Gao, Jingli Gu, Lan Zhou, Shu Guo, Wei Hao, Zhuan Zhou, Ji-Min Cao. (2012) Nerve sprouting contributes to increased severity of ventricular tachyarrhythmias by upregulating iGluRs in rats with healed myocardial necrotic injury. J Mol Neurosci, 48:448–455

64. Huang HP, Zhu FP, Chen XW, Xu ZQD, Zhang CX and Zhou Z（2012）Physiology of quantal norepinephrine release from somatodendritic sites of neurons in locus coeruleus. Frontiers in Molecular Neuroscience, 5(29):1-5

63. Guo N, Yao W, Wang SR , Zhu J, Huang D, Zuo PL, Kang XJ, Fu CL, Zhou Z*, Zhang B* (2012) Nicotine dynamically modulates dopamine clearance in rat striatum in vivo. Neurochemistry International, 60: 55-359

62. Dou HQ, Xu YF, Sun JP, Shang SJ, GuoS, Zheng LH, Chen CC, Bruce IC, Yu X, and Zhou Z (2012) Thiopental-induced insulin secretion via activation of IP3-sensitive calcium stores in rat pancreatic beta cells. American Journal of Physiology-Cell, 302:C796-C803

61. Zhang B, Zhang XY, Huang W, Luo PF, Zhu FP, Liu T, Du YR, Wu QH, Lü J, Xiu Y, Liu LN, Huang HP, Guo S, Zheng H, Zhang CX, and Zhou Z (2012) Action potential triggered somatic exocytosis in mesencephalic trigeminal nucleus neurons in rat brain slices. Journal of Physiology, 590: 753–762.

60. Wang SR, Yao W, Huang HP, Zhang B, Zuo PL, Sun L, Dou HQ, Li Q, Kang XJ, Xu HD, Hu MQ, Jin M, Zhang L, Mu Y, Peng JY, Zhang CX, Ding JP, Li BM, Zhou Z (2011) Role of vesicle pools in action potential pattern-dependent dopamine overflow in rat striatum in vivo. Journal of Neurochemistry, 119:342–353

59. Xiong W, Liu T, Wang YS, Chen XW, Sun L, Guo N, Zheng H, Zheng LH, Ruat M, Han WP, Zhang CX, and Zhou Z (2011) Extracellular Ca2+ directly inhibits neuronal exocytosis in cell body. PloS One, 6: e24573

58. Tao Liu, Lei Sun, Yingfei Xiong, Shujiang Shang, Ning Guo, Sasa Teng, Yeshi Wang, Bing Liu, Changhe Wang, Li Wang, Lianghong Zheng, Claire Xi Zhang, Weiping Han, and Zhuan Zhou (2011) Calcium triggers quantal release simultaneously from two types of vesicles in a single astrocyte. Journal of Neuroscience，31:10593–10601.

57. Tao Liu, Shu-Jiang Shang, Bin Liu, Chang-He Wang, Ye-Shi Wang, Wei Xiong, Liang-Hong Zheng, Claire Xi Zhang, and Zhuan Zhou (2011) Two distinct vesicle pools for depolarization-induced exocytosis in somata of dorsal root ganglion neurons, Journal of Physiology, 589:3507–3515.

56. Bo Zhang, Liang Sun, Yi-Mei Yang, Hong-Ping Huang, Fei-Peng Zhu, Li Wang, Xiao-Yu Zhang, Shu Guo, Pan-Li Zuo, Claire X. Zhang, Jiu-Ping Ding, Lu-Yang Wang, and Zhuan Zhou (2011) Action potential bursts enhance transmitter release at a giant central synapse. Journal of Physiology，589:2213–2227.

55. Zhang Y, Su P, Liang P, Liu T, Liu X, Liu XY, Zhang B, Han T, Zhu YB, Yin DM, Li JF, Zhou Z, Wang KW and Wang Y (2010) The DREAM Protein Negatively Regulates the NMDA Receptor through Interaction with the NR1 Subunit. Journal of Neuroscience, 30(22):7575–7586

54. Huang H, Wei ZW, Huang YY, Zhao DY, Zheng LY, Cai TJ, Wu MX, Wang W, Ding XF, Zhou Z, Du Q, Li ZH and Liang ZC (2010) An efficient and high-throughput electroporation microchip applicable for siRNA delivery. Lab on a Chip, 11:163-172

53. Huang H, Wei N, Xiong YF, Yang F, Fang HQ, Xie WJ, Zhou Z, Cheng HP, Liang ZC, Du Q (2010) Exogenous nucleic acids aggregate in non-P-body cytoplasmic granules when transfected into cultured cells. Front Biol, 5(3):272-281.

52. Zheng H, Fan J, Xiong W, Zhang C, Wang XB, Liu T, Liu HJ, Sun L, Wang YS, Zheng LH, Wang BR, Zhang CX, Zhou Z (2009) Fractional contributions of Ca-dependent and Ca-independent secretion induced by action potentials in single sensory neurons. Biophysical Journal. 96:2449–2456

51. Misler S, Zhou Z, Dickey AS, Silva AM, Pressel DM, Barnett DW (2009) Biphasic insulin secretion from ?-cells of canine islets of Langerhans: tuning two modes of exocytosis to two modes of glucose-induced electrical activity?, Channels, 3(3): 181 - 193

50. Fan J, Zhang B, Shu HF, Zhang XY, Wang X, Kuang F, Liu L, Peng ZW, Wu R, Zhou Z, Wang BR (2009) IL-6 Increases [Ca2+]i Level and Induces Catecholamine Secretion in the Rat Carotid Body Glomus Cells. Journal of Neuroscience Research, 87(12):2757-62

49. Li B, Chen F, Ye JN, Chen XW, Yan J, Li Y, Xiong Y, Zhou Z, Xia JX and Hu ZA（2009）The Modulation of Orexin A on HCN Currents of Pyramidal Neurons in Mouse Prelimbic Cortex. Cerebral Cortex, 20:1756-1767

48. Chen XW, Feng YQ, Hao CJ, Guo XL, He X, Zhou ZY, Guo N, Huang HP, Xiong W, Zheng H, Zuo PL, Zhang CX, Li W, Zhou Z（2008）A Schizophrenia-Susceptible Gene Affects Quanta Size of Transmitter Release. J Cell Biol, 181: 791-801

47. Chen XW, Mu Y, Huang HP, Guo N, Zhang B, Fan SY, Xiong JX, Wang SR, Xiong W, Huang W, Liu T, Zheng LH, Zhang CX, Li LH, Yu ZP, Hu ZA, Zhou Z (2008) Hypocretin-1 potentiates NMDA receptor-mediated somatodendritic secretion from locus coeruleus neurons. J. Neurosci., 28:3202-08.

46. Chen XW, Huang W, Yan JA, Fan HX, Guo N, Lü J, Xiu Y, Gu JL, Zhang CX, Ruan HZ, Hu ZA, Yu ZP, Zhou Z (2008) Reinvestigation of the effect of orexin A on catecholamine release from adrenal chromaffin cells. Neuroscience Letters, 436:181-184.

45. Huang HP, Wang SR, Yao W, Zhang C, Zhou Y, Zhang B, Xiong W, Chen XW, Zheng LH, Wang LY, Marc Landry, Hokfelt T, Xu ZQD and Zhou Z (2007) Long latency of evoked quantal transmitter release from somata of locus coeruleus neurons in rat pontine slices. Proc Natl Acad Sci U S A., 104(4):1401-1406

44. Zhang W, Zhang Y, Zheng H, Zhang C, Xiong W, Olyarchuk JG, Walker M, Xu W, Zhao M, Zhao S, Zhou Z*, Wei L*. (2007) SynDB: A Synapse protein DataBase based on Synapse Ontology. Nucl. Acid Res., 35: D737–D741.

43. Shu HF, Wang BR, Wang SR, Yao W, Huang HP, Zhou Z, Wang X, Fan J, Wang T, Ju G. (2007) IL-1beta inhibits IK and increases [Ca2+]i in the carotid body glomus cells and increases carotid sinus nerve firings in the rat. Eur J Neurosci. 25(12):3638-47.

42. Yu X, Chen XW, Zhou P, Yao LJ, Liu T, Zhang B, Li Y, Zheng H, Zheng LH, Zhang CX, Bruce I, Ge JB, Wang SQ, Hu ZA, Yu HG and Zhou Z (2007) Calcium influx through If channels in rat ventricular myocytes. Am J Physiol-Cell. Am J Physiol-Cell, 292: C1147-1155.

41. Yao LJ, Wang G, Ou-Yang KF, Wei CL, Wang XH, Wang SR, Yao W, Huang HP, Luo JH, Wu CH, Liu J, Zhou Z*, Cheng HP*（2006）Ca2+ sparks and Ca2+ glows in superior cervical ganglion neurons. Acta Pharmacologica Sinica, 27 (7): 848–852

40. Chen XK, Xiong YF, and Zhou Z (2006) “Kiss-and-Run” Exocytosis in Astrocytes. Neuroscientist, 12(5): 375–378.

39. Wang LC, Xiong W, Zheng J, Zhou Y, Zheng H, Zhang C, Zheng LH, Zhu XL, Xiong ZQ, Wang LY, Cheng HP, Zhou Z (2006) The timing of endocytosis following activation of a G-protein-coupled receptor in a sensory neuron. Biophysical Journal, 90:3590-3598

38. Chen XK, Wang LC, Zhou Y, Zheng LH and Zhou Z (2005) “Kiss-and-Run” Glutamate Secretion in Cultured and Freshly Isolated Rat Hippocampal Astrocytes J. Neurosci., 25(40):9236 –9243

a) Highlighted in Nature Reviews Neuroscience (2005) DIO 10.1038. （http://www.nature.com/nrn/journal/v6/n12/full/nrn1815.html）

37. Chen XK, Wang LC, Zhou Y, Cai Q, Prakriya M, Duan KL, Sheng ZH, Lingle C, Zhou Z (2005) Activation of GPCRs modulates quantal size in chromaffin cells through Gbc and PKC. Nature Neuroscience, 8(9):1160 - 1168.

36. Ouyang K, Zheng H, Qin X, Zhang C, Yang D, Wang X, Wu C, Zhou Z*, Cheng H* （2005）Ca2+ Sparks and Secretion in Dorsal Root Ganglion Neurons. Proc Natl Acad Sci U S A., 102:12259-64.

35. Yang H, Zhang C, Zheng H, Xiong W, Zhou Z, Xu T, Ding JP (2005) A simulation study on the Ca2+-independent but voltage-dependent exocytosis and endocytosis in dorsal root ganglion neurons. Eur. Biophy. J., 34:1007-16

34. Yao J, Chen X, Li H, Zhou Y, Yao L, Wu G, Chen X, Zhang N, Zhou Z, Xu T, Wu H, Ding J (2005) BmP09, a ‘Long-Chain’ Scorpion Peptide Blocker of BK Channels. J. Biol. Chem. 280(15):14819-28

33. Wu B, Wang YM, Xiong W, Zheng LH, Fu CL, Bruce I, Zhang C and Zhou Z (2005) Optimization of a multi-channel puffer system for rapid delivery of solutions during patch-clamp experiments. Frontiers in Bioscience. 10:761-7

32. Xiao RP, Cheng HP, Zhou Z, Chen YQ, Wu CH & Gu XC (2004) Can China Join in main stream of Molecular Medicine? Nature, 432: A44-47

31. Xu CQ, He LL, Brone B, Martin-Eauclaire MF, Van Kerkhove E, Zhou Z, Chi CW. (2004) A novel scorpion toxin blocking small conductance Ca2+ activated K+ channel. Toxicon. 43, 961-71.

30. Zhang C, Xiong W, Zheng H, Wang L, Lu B and Zhou Z (2004) Calcium- and dynamin-independent endocytosis in dorsal root ganglion neurons. Neuron, 42: 225–236

29. Yu X, Duan KL, Shang CF, Yu HG and Zhou Z (2004) Calcium influx through hyperpolarization-activated cation channels ( Ih channels) contributes to activity-evoked neuronal secretion. Proc Natl Acad Sci U S A., 101:1051-1056.

28. Duan KL, Yu X, Zhang C, and Zhou Z (2003) Control of Secretion by Temporal Patterns of Action Potentials in Adrenal Chromaffin Cells. J. Neurosci., 23(35):11235-43

27. Lou XL, Yu X, Chen XK, Duan KL, He LM, Qu AL, Xu T, Zhou Z (2003) Na channel inactivation: a comparison study between pancreatic islet ?-cells and adrenal chromaffin cells in rat. J. Physiol (Lond) 548: 191-202.

26. Fan CX, Chen XK, Zhang C, Wang LX, Duan KL, He LL, Cao Y, Liu SY, Zhong MN, Ulens C, Tytgat J, Chen JS, Chi CW, Zhou Z (2003) A Novel Conotoxin from Conus betulinus, ?-BtX, unique in Cysteine Pattern and in Function as a specific BK Channel Modulator. J. Biol. Chem. 278:12624-33

25. Bao L, Jin SX, Zhang C, Wang LH, Xu ZZ, Zhang FX, Wang LC, Ning FS, Cai HJ, Guan JS, Xiao HS, Xu ZQ, He C, Hokfelt T, Zhou Z*, Zhang X* (2003) Activation of delta-Opioid Receptors on Dorsal Root Ganglion Neurons Induces Receptor Insertion and Neuropeptide Secretion. Neuron, 37:121-133.

24. Ji YH, Wang WX, Ye JG, He LL, Li YJ, Yan YP, Zhou Z (2003) BmTX3, a Novel Specific Blocker of Ca2+-Activated K+ Channel from Asian Scorpion Venom: Purification, Genomic Organization and Function Assessment. J. Neurochem. 84(2):325-35.

23. Song P, Lie-Cheng W, Wang GD, Zhou Z, Zhao ZQ (2003) Interleukin-2 regulates calcium current and intracellular calcium via Mu opioid receptors in rat dorsal root ganglion neurons. Neuropharmacology, 43(8):1324-1329

22. He LM, Chen LY, Lou XL, Qu AL, Zhou Z, Xu T. (2003) Rapid inhibitory effects of corticosterone on calcium influx rat dorsal root ganglion neurons. Neuroscience, 116(2):325-33.

21. Zhou Z*, Bers DM (2002) Time Course of Mitochondrial Antagonists Blockade in Intact Cells. European Journal of Physiology, 445:132-138

20. Wu JJ, He LL, Zhou Z, and Chi ZW (2002) Gene Expression, Mutation and Structure-Function Relationship of Scorpion Toxin BmP05 Active on SKCa Channels. Biochemistry, 41(8):2844-9

19. Zhang C & Zhou Z (2002) Ca2+-independent but voltage-dependent secretion in mammalian dorsal root ganglion neurons. Nature Neuroscience, 5(5):425-30

18. He L, Chen L, Lou X, Qu A, Zhou Z, Xu T. (2002) Evaluation of beta-amyloid peptide 25-35 on calcium homeostasis in cultured rat dorsal root ganglion neurons. Brain Res 939(1-2):65-75

17. Lou XL, Zeng, XH, Qu AL and Zhou Z (2001) Glucose induced Ca signals in rat pancreatic ? cells. Progress in Natural Sciences, 11:109-114 (Chinese version: 10:812-817)

16. Zhou.Z & Bers.DM (2000) Ca influx via L-type Ca channel at voltages positive to the reversal potential in ventricular myocytes. Journal of Physiology (London) 523: 57-66

15. Zeng XH, Qu AL, Lou XL, Xu JH, Wang JJ, Wu HX and Zhou Z (2000) Ca signals induced from calcieum stores in pancreatic islets ?-cells. Chinese Science Bulletin, 45:51-56 (Chinese version: 44:2058-2063)

14. Zhou.Z , Matlib.MA & Bers.DM (1998) Cytosolic and mitochondrial Ca2+ signals in patch clamped mammalian ventricular myocytes. Journal of Physiology (London), 507：379-403

13. Matlib MA, Zhou Z, Knight S, Ahmed S, Choi KM, Krause-Bauer J, Phillips R, Altschuld R, Katsube Y, Sperelakis N, Bers DM (1998) Oxo-Bridged Dinuclear ruthenium Ammine Complex Specifically inhibits Ca2+ Uptake into mitochendria in vitro and in situ in single cardiac myocytes. J. Biol. Chem.273(17):10223-31,

12. Elhamdani A, Zhou Z, Arttalejo CR (1998) Timing of dense-core vesicle exocytosis depends on the facilitation L-type Ca channel in adrenal chromaffin cells. J. Neurosci. 18(16):6230-40

11. Wei Shun-hui, Anlian Qu, Hongxiu Wu, Zhuan Zhou (1998). A kinetic model of control on secretion in neuroendocrine cells. IEEE/EMBS, 20: 3083-3086

10. Zhou.Z, Misler.S & Chow .RH (1996) Rapid fluctuations in transmitter release from single vesicles in bovine adrenal chromaffin cells. Biophysical Journal, 70:1543-52

9. Zhou.Z, Misler.S (1996) Amperometric detection of quantal secretion from patch-clamped rat pancreatic beta-cells. J Biol Chem 271(1):270-7

8. Zhou.Z, Misler.S (1995) Amperometric detection of stimulus-induced quantal release of catecholamines from cultured superior cervical ganglion neurons. Proc Natl Acad Sci USA 92(15):6938-42

7. Burnashev, N., Zhou, Z., Neher, E. & Sakmann, B. (1995) Calcium flux and fractional calcium currents through recombinant GluR channels of NMDA-R, AMPA-R and KA-R Subtypes. J. Physiol. (London), 485:403-418

6. Zhuan Zhou & Stanley Misler (1995) Action potential induced catecholamine secretion in rat chromaffin cells. Journal of Biological Chemistry, 270: 3498-3505.

5. Zhuan Zhou & Erwin Neher (1993). Mobile and immobile Ca buffers in bovine adrenal chromaffin cells. J. Physiol. (London). 469: 245-273

4. Schneggenburger,R., Zhou,Z., Konnerth,A. & Neher,E. (1993). Fractional contribution of calcium to the cation current through glutamate receptors. Neuron, 11: 133-143

3. Zhuan Zhou & Erwin Neher (1993). Calcium permeability of nicotinic acetylcholine receptor channels in bovine adrenal chromaffin cells. Pflugers Arch, 425: 511-517.

2. Zhuan Zhou & Huaguang Kang (1990) patch clamp noise from seal impedance and the pipette capacitance. Proc. IEEE/EMBS, 12(4):1679-1680.

1. H.G. Kang & Zhuan Zhou (1990) Low noise design of patch clamp amplifier, Proc. IEEE/EMBS, 12(3):1103-1104. 

 

Patents

1. Zhuan Zhou* and He-Ping Cheng (2006): "METHODS AND SYSTEMS FOR SCREENING LIGAND-RECEPTOR BINDING in living cells" (USA patent No. 200610026241.9; Chinese patent) （*correspondence author）

2. Mu-Min Poo, Zhuan Zhou*, Shu-Ming Duan, Xin-Qiao He and Qian Hu (2004): " A X-Y stage for combined confocal microscopy electrophysiology"， Chinese patent (发明专利) ZL 02112182.6 (*correspondence author)

3. Zhuan Zhou (2003): "A magnetic button for wiring on stage". Chinese patent (实用新型) ZL 02216336.0