汪阳明 教授

Ph.D.博士生导师

干细胞与非编码核酸研究室主任

联系电话：010-62766945

E-mail: ymw129@gmail.com

通讯地址：缅甸腾龙国际官网腾龙娱乐app官网下载研究所 100871
 

汪阳明教授于2000年本科毕业于缅甸腾龙国际官网生物技术系, 获理学学士学位，2006年获美国伊利诺斯大学(Urbana-Champaign)生物化学专业博士学位。2006-2010年在加州大学（San Francisco）从事博士后研究，现任缅甸腾龙国际官网腾龙娱乐app官网下载研究所全职教授。

主要学术业绩：主要从事非编码小核酸在胚胎干细胞中功能的研究。建立microRNA 全敲除小鼠胚胎干细胞，率先证明了microRNA 作为一个群体对于胚胎干细胞增殖与分化具有重要功能，从而为这个领域的进一步研究指明了方向。 随后，利用这些microRNA 敲除小鼠胚胎干细胞，发展了一套筛选microRNA功能的新方法， 并发现了调控胚胎干细胞的增殖与细胞周期的重要microRNA 簇。 这个发现直接启发了其它与此microRNA簇相关的功能的研究， 包括它们对胚胎干细胞全能性以及体细胞重编程成为诱导多能干细胞过程中的作用。Steven Dalton在Cell Stem Cell撰文认为我的研究揭示了胚胎干细胞周期调控的全新一页。 另外，利用高通量测序技术和生物信息学，在小鼠胚胎干细胞中发现并定量测定了若干以前不为人所知的小核酸，为进一步研究它们的功能和生物合成奠定了基础。此期间在Nature Genetics上发表第一作者论文两篇， Genes and Development 上发表署名作者论文一篇。被邀请为Cancer Research撰写综述，并参与编写专著一部。

主要研究方向: 利用分子生物学, 细胞生物学,以及动物模型来研究非编码核酸在干细胞的自我更新与分化过程中的功能及其作用机理。

代表论文：

ORIGINAL PAPERS

14. Y. Wang, S. Baskerville, A. Shenoy, J.E. Babiarz, L. Baehner and R. Blelloch, “Embryonic Stem Cell Specific microRNAs Regulate the G1/S Transition and Promote Rapid Proliferation”, Nature Genetics 2008, 40, 1478-1483. (Highlighted in Nature Reports Stem Cells; featured in News and Views in Nature Genetics, 2008, 40, 1391-1392; previewed in Cell Stem Cell, 2009, 4, 9-10)

13. J.E. Babizrz, J.G. Ruby, Y. Wang, D.P. Bartel, and R. Blelloch, “Endogenous shRNAs, siRNAs, and Other Microprocessor-independent, Dicer-dependent Small RNAs in Mouse ES cells”, Genes & Development 2008, 22, 2773-2785.

12. Y. Wang, R. Medvid, C. Melton, R. Jaenisch, and R. Blelloch, “DGCR8 is Essential for microRNA Biogenesis and Silencing of Embryonic Stem Cell Self-Renewal”, Nature Genetics 2007, 39, 380-385.

11. Y. Wang and S.K. Silverman, “Experimental Tests of Two Proofreading Mechanisms for 5′-Splice Site Selection”, ACS Chemical Biology 2006, 1, 316-324.

10. Y. Wang and S. K. Silverman, “Efficient RNA 5'-Adenylation By T4 DNA Ligase to Facilitate Practical Applications”, RNA 2006, 12, 1142-1146.

9. E. Zelin, Y. Wang and S.K. Silverman, “Adenosine is Inherently Favored as the Branch-Site RNA Nucleotide in a Structural Context that Resembles Natural RNA Splicing”, Biochemistry 2006, 45, 2767-2771.

8. Y. Wang and S. K. Silverman, “A General Two-Step Strategy To Synthesize Lariat RNAs”, RNA 2006, 12, 313-321.

7. Y. Wang and S. K. Silverman, “Efficient One-Step Synthesis of Biologically Related Lariat RNAs by a Deoxyribozyme”, Angewandte Chemie International Edition 2005, 44, 5863-5866. (Highlighted in Nature Chemical Biology, 2005, 1, 195)

6. E. D. Pratico, Y. Wang, and S. K. Silverman, “A Deoxyribozyme That Synthesizes 2',5'-Branched RNA With Any Branch-Site Nucleotide”, Nucleic Acids Research 2005, 33, 3503-3512.

5. Y. Wang and S. K. Silverman, “Directing the Outcome of Deoxyribozyme Selections to Favor Native 3'-5' RNA Ligation”, Biochemistry 2005, 44, 3017-3023.

4. Y. Wang and S. K. Silverman, “Characterization of Deoxyribozymes that Synthesize Branched RNA”, Biochemistry 2003, 42, 15252-15263.

3. Y. Wang and S. K. Silverman, “Deoxyribozymes that Synthesize Branched and Lariat RNA”, Journal of the American Chemical Society 2003, 125, 6880-6881.

2. A. Flynn-Charlebois, Y. Wang, T. K. Prior, I. Rashid, K. A. Hoadley, R. L. Coppins, A. C. Wolf, and S. K. Silverman, “Deoxyribozymes with 2'-5' RNA Ligase Activity”, Journal of the American Chemical Society 2003, 125, 2444-2454.

1. W. Zhen, Y. Wang, W. Ding, X. Chen, Y. Hu, and Z. Lin, “Cre/loxP-mediated Deletion of Transgene in Transgenic Tobacco”, Journal (natural sciences) of Peking Univeristy 2001, 37, 477-482.

INVITED REVIEW

Y. Wang and R. Blelloch, “Cell cycle regulation by microRNAs in embryonic stem cells”, Cancer Research 2009, 69, 4093-4096.